microarray hybridization buffer ii  (Thermo Fisher)

Journal: Virus research

Article Title: Differential gene expression related to Nora virus infection of Drosophila melanogaster

doi: 10.1016/j.virusres.2013.03.021

Figure Lengend Snippet: Genes identified by cDNA microarray analysis as up-regulated by 1.5 fold or greater in Nora virus infected flies as compared to uninfected flies with a p -value of less than 0.01. The fold change was determined based on the average results of 3 microarrays and the gene names, gene ID #, and gene biological functions were identified using Flybase and NCBI GenBank.

Article Snippet: Fragmented probes were added to 40 µl of Ambion Microarray Hybridization Buffer II and denatured by heat at 95 °C for 5 min and kept at 60 °C until hybridization.

Techniques: Microarray, Infection, Activity Assay, Binding Assay

Journal: Virus research

Article Title: Differential gene expression related to Nora virus infection of Drosophila melanogaster

doi: 10.1016/j.virusres.2013.03.021

Figure Lengend Snippet: Genes identified by cDNA microarray analysis as down-regulated by 1.5 fold or greater in Nora virus infected flies as compared to uninfected flies with a p -value of less than 0.01. The fold change was determined based on the average results of 3 microarrays and the gene names, gene ID #, and gene biological functions were identified using Flybase and NCBI GenBank.

Article Snippet: Fragmented probes were added to 40 µl of Ambion Microarray Hybridization Buffer II and denatured by heat at 95 °C for 5 min and kept at 60 °C until hybridization.

Techniques: Microarray, Infection, Activity Assay, Binding Assay

Journal: Virus research

Article Title: Differential gene expression related to Nora virus infection of Drosophila melanogaster

doi: 10.1016/j.virusres.2013.03.021

Figure Lengend Snippet: Initial validation of microarray data by qRT-PCR analysis of one up-regulated (Cp16) and one down-regulated (TpnC4) gene. The light gray bars represent the mRNA levels quantified by cDNA microarray and the black bars represent the mRNA levels quantified by qRT-PCR analysis. The data from both analyses is consistent. The error bars represent the standard error of the mean and n = 3.

Article Snippet: Fragmented probes were added to 40 µl of Ambion Microarray Hybridization Buffer II and denatured by heat at 95 °C for 5 min and kept at 60 °C until hybridization.

Techniques: Microarray, Quantitative RT-PCR

Journal: Virus research

Article Title: Differential gene expression related to Nora virus infection of Drosophila melanogaster

doi: 10.1016/j.virusres.2013.03.021

Figure Lengend Snippet: Independent infection experiment to validate microarray data by qRT-PCR analysis of three up-regulated (Cp16, Fcp3c, and BtbVII) and two down-regulated (TpnC4 and CG6639) genes. The light gray bars represent the mRNA levels quantified by cDNA microarray and the black bars represent the mRNA levels quantified by qRT-PCR analysis. Cp16 and TpnC4 are shown first because they were used in the initial validation and in this experiment to provided consistency and direct data comparison. The data from the cDNA microarray and qRT-PCR analyses is consistent, as are the results between the initial validation and this subsequent experiment. The error bars represent the standard error of the mean and n = 3.

Article Snippet: Fragmented probes were added to 40 µl of Ambion Microarray Hybridization Buffer II and denatured by heat at 95 °C for 5 min and kept at 60 °C until hybridization.

Techniques: Infection, Microarray, Quantitative RT-PCR